Recombinant:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
Magnetic Cell Separation:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
Purification:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
Transfection:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
Marker:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
Membrane:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
Enzyme-linked Immunosorbent Assay:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
Magnetic Beads:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
Luciferase:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
Polymerase Chain Reaction:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
DNA Ligation:Article Title: Guanylate-Binding Proteins 2 and 5 Exert Broad Antiviral Activity by Inhibiting Furin-Mediated Processing of Viral Envelope Proteins.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: lymphoid aggregate cultures (HLACs) Bundeswehr Hospital Ulm, Germany N/A Chemicals, Peptides, and Recombinant Proteins L-Glutamine Pan Biotech Cat# P04-80100 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 Fungizone Thermo Fisher Cat# 15290026 Non-Essential Amino Acids Solution Thermo Fisher Cat# 11140050 Sodium Pyruvate Thermo Fisher Cat# 11360070 Gentamicine Sulfate Fisher Scientific Cat# 30-005-CR Timentin GlaxoSmithKline Cat# NDC 0029-6571-26 GSK Human Serum Sigma-Aldrich Cat# H4522-100ML Human M-CSF R&D Systems Cat# 216-MC-01M Human IL-2 IS, premium grade MACS Miltenyi Biotec Cat#130-097-745 Remel PHA purified Thermo Fisher Cat# R30852801 Human Interferon a 2a pbl assay science Cat# 11101-1 IFNa subtypes This paper N/A Human Interferon g Sigma-Aldrich Cat# I3265 Dynabeads Human T-Activator CD3/CD28 Thermo Fisher Cat# 111.32D Biocoll Separation Solution Biochrom Cat# L6115 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Cat# 13778150 TransIT LT-1 Mirus Bio Cat# MIR2305 4X Protein Sample Loading Buffer LI-COR Cat# 928-40004 2-Mercaptoethanol Sigma-Aldrich Cat# M6250-100ML BlueStar Plus Prestained Protein Marker Nippon genetics Cat# MWP04 NuPAGE 4-12% Bis-Tris Protein Gels Invitrogen Cat# NP0323BOX Cat# WG1403BOX PVDF membrane Merck Milipore Cat# IPFL00010 Hoechst 33342 Solution Thermo Fisher Cat# #62249 Phalloidin Atto-647N ATTO-TEC Cat# AD647N-81 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 NucRed Live 647 ReadyProbes Reagent Thermo Fisher Cat# R37106 Pyr-RTKR-AMC Bachem Cat# I-1650 HIV-1 p24 protein (ELISA standard) Abcam Cat# 43037 KPL SureBlue TMB Microwell Peroxidase Substrate Medac Cat# 52-00-04 Trypsin from bovine pancreas, TPCK treated Sigma-Aldrich Cat# T1426 Avicel RC 581 FMC Biopolymers Free sample Pierce Protein A/G Magnetic Beads Thermo Fisher Cat# 88802 2-(4-Methylumbelliferyl)- a-D-N- acetylneuraminic acid sodium salt (MUNANA substrate) GOLDBIO Cat# M-520-25 Critical Commercial Assays GalScreen Applied Biosystems Cat# T1027 RosetteSep Human CD4+ T Cell Enrichment Cocktail Stem Cell Technologies Cat# 15062 Luciferase Assay System Promega Cat# E1501 FIX & PERM Kit Nordic-MUbio Cat# GAS-002-1 Pierce HA Tag IP/CoIP Kit Thermo Fisher Cat# 26180 Phusion high-fidelity PCR Kit Thermo Fisher Cat# F553S DNA Ligation Kit Ver. .. 2.1 TaKaRa Cat# 6022 (Continued on next page) e2 Cell Reports 27, 2092–2104.e1–e10, May 14, 2019
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